Name : Qing X Li
Title : Professor
Unit : Department of Molecular Biosciences & BioEngineering
Address : 1955 East-West Rd. #218 Honolulu, HI 96822
Room : Ag Sciences Building 402J
Phone : (808)956-2011
Fax : (808)956-3542
E-mail : qingl@hawaii.edu
Website : http://web.hawaii.edu/lilab/
Specialties :
Proteomics; Agricultural chemistry and biochemistry; Environmental fate of agrochemicals; Bioremediation; Food safety; Functional foods; Alzheimer's disease drug discovery
Professional Prep/Appointments :
B.S. Agriculture, 1982, Agricultural University of Shandong, China
Ph.D. Agricultural and Environmental Chemistry, 1990, University of California at Davis
Post-doctoral fellow, 1991-1994, University of California at Berkeley
Projects :
1. Palm peroxidases. We isolate, clone, engineer and express palm peroxidases for biosynthesis of conductive polymers, biosensing and remediation. We study plant glycoprotein heterogeneity, glycoforms and structure and activity relationship.
2. Botanical insecticides. We search for novel bioinsecticides and elucidate their mode of action.
3. Functional foods and pharmaceuticals. We isolate and characterize natural products for Alzheimer's disease and cancer prevention.
4. Food safety, environmental fate and biomonitoring of agrochemicals. We study pesticides and pollutants in foods and the environment. We develop new immunoassays and other analytical methods. We study gas chromatography separation theory, elution model development and computational simulation.
5. Microbial stress responses to toxic chemicals and mechanisms of biotransformation. We isolate novel bacteria, Archaea and fungi for bioremediation of pollutants and biosynthesis of high value products. We also study biocontamination of biofuels and blended fuels.
6. Proteomics. We study glycoproteomics and phosphoproteomics.
Courses:
Environmental Biochemistry (MBBE412, 3 credits- lecture course)
Advanced Laboratory Techniques (MBBE687, 3 credits- lecture/lab course; Proteomics-focused)
Research Interests :
Our research focuses on agricultural and environmental chemistry and biochemistry in five areas. Our research addresses problems relevant to agrochemicals and foods for human and environment health. Proteomics is used as a tool for the studies.
1. Palm peroxidases. We isolate, clone, engineer and express palm peroxidases for biosynthesis of conductive polymers, biosensing and remediation. We study plant glycoprotein heterogeneity, glycoforms and structure and activity relationship.
2. Botanical insecticides. We search for novel bioinsecticides and elucidate their mode of action.
3. Functional foods and phamaceuticals. We isolate and characterize natural products for Alzheimer's disease and cancer prevention.
4. Food safety, environmental fate and biomonitoring of agrochemicals. We study pesticides and pollutants in foods and the environment. We develop new immunoassays and other analytical methods. We study gas chromatography separation theory, elution model development and computational simulation.
5. Microbial stress responses to toxic chemicals and mechanisms of biotransformation. We isolate novel bacteria, Archaea and fungi for bioremediation of pollutants and biosynthesis of high value products. We also study biocontamination of biofuels and blended fuels.
Selected Publications :
Please see: Web: http://web.hawaii.edu/lilab/
Web: http://manoa.hawaii.edu/jabsom/proteomics
Google scholar: https://scholar.google.com/citations?user=6JswiOgAAAAJ&hl=en
Publications selected out of 340 papers to reflect what topics of research we do
337. Fu, B.; Baker, M.R.; Li, Q.X. Effect of N-linked glycosylation of recombinant windmill palm tree peroxidase on its activity and stability. Journal of Agricultural and Food Chemistry. Doi: 10.1021/acs.jafc.8b00234.
334. Doello, S.; Liang, Z.; Cho, I.K.; Kim, J.B.; Li, Q.X. Cytotoxic effects of 24-methylenecyloartanyl ferulate on A549 non-small cell lung cancer cells through MYBBP1A up-regulation and AKT and Aurora B kinase inhibition. Journal of Agricultural and Food Chemistry. Doi: 10.1021/acs.jafc.8b00491.
332. Liang, Z.; Li, Q.X. 2018. π-cation interactions in molecular recognition: Perspectives on pharmaceuticals and pesticides. Journal of Agricultural and Food Chemistry Doi: 10.1021/acs.jafc.8b00758.
331. Liang, Z.; Li, Q.X. Discovery of potent, selective, substrate-competitive and bioavailable glycogen synthase kinase-3β inhibitors for Alzheimer’s disease: design, synthesis and biological evaluation of novel C-glycosylflavones. ACS Chemical Neuroscience Doi: 10.1021/acschemneuro.8b00010.
327. Wang, Y.; Na, R.; Zhu, M.; Jiang, E.; Wang, L.; Fan, S.; Wang, Z.; Li, Q.X.; Hua, R. 2018. A colorimetric and ratiometric dual-site fluorescent probe with 2,4-dinitrobenzenesulfonyl and aldehyde groups for imaging of aminothiols in living cells and zebrafish.Dyes and Pigments 156: 338-347.
325. Wang, Y.; Liu, J.; Zhu, M.; Wang, L.; Zen, X.; Fan, S.; Wang, Z.; Li, H.; Na, R.; Zhao, X.; Li, Q.X. 2018. Biophysical characterization of interactions between falcarinol-type polyacetylenes and human serum albumin via multispectroscopy and molecular docking techniques. Journal of Luminescence 200: 111-119. Doi: 10.1016/j.jlumin.2018.03.082.
324. Sanchis, A.; Salvador, J.-P.; Campbell, K.; Elliott, C.T.; Shelver, W.L.; Li, Q.X.; Marco, M.-P. 2018. Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples. Talanta 184: 499-506. DOI.org/10.1016/j.talanta.2018.03.036.
322. Chu, S.; Baker, M.R.; Leong, G.; Letcher, R.J.; Li, Q.X. Covalent binding of the organophosphate insecticide profenofos to tyrosine on α- and β-tubulin proteins. Chemosphere 199: 154-159. 10.1016/j.chemosphere.2018.02.003.
321. Zhang, L.; Zhou, L.; Li, Q.X.; Liang, H.; Qin, H.; Masutani, S.; Yoza, B. 2018. Toxicity of lanthanum oxide nanoparticles to the fungus Moniliella wahieum Y12T isolated from biodiesel. Chemosphere 199: 495-501. DOI: 10.1016/j.chemosphere.2018.02.032.
320. Fu, B.; Xu, T.; Cui, Z.; Ng, Ho, L.; Wang, K.; Li, J.; Li, Q.X. 2018. Mutation of phenylalanine-223 to leucine enhances transformation of benzo[a]pyrene by ring-hydroxylating dioxygenase of Sphingobium sp. FB3 by increasing accessibility of the catalytic site.Journal of Agricultural and Food Chemistry 66(5), 1206-1213. DOI: 10.1021/acs.jafc.7b05018
319. Lv, P.; Chen, Y.; Zhao, Z.; Shi, T.; Wu, X.; Li, Q.X.; Hua, R. 2018. Design, synthesis and antifungal activities of 3-acyl thiotetronic acid derivatives: New fatty acid synthase inhibitors. Journal of Agricutlrual and Food Chemistry 66(4): 1023–1032. DOI: 10.1021/acs.jafc.7b05491.
318. Islam, F.; Wang, J.; Farooq, M.A.; Khan, M.S.S.; Xu, L.; Zhu, J.; Zhao, M.; Muños, S.; Li, Q.X.; Zhou, W. 2018. Potential impact of the herbicide 2,4-dichlorophenoxyacetic acid on human and ecosystems. Environmental International 111: 332-351. DOI: 10.1016/j.envint.2017.10.020. PMID: 29203058.
317. Sun, L.; Pan, D.; Liu, J.; Wu, X.; Hua, R.; Li, Q.X. 2018. Leucobacter prometrynivorans sp. nov., a prometryn-degrading bacterium isolated from sludge. International Journal of Systematic and Evolutionary Microbiology 68: 204-210. DOI: 10.1099/ijsem.0.002483
313. Liu, J.; Pan, D.; Wu, X.; Chen, H.; Cao, H.; Li, Q.X.; Hua, R. 2018. Enhanced degradation of prometryn and other s-triazine herbicides in pure cultures and wastewater by polyvinyl alcohol-sodium alginate immobilized Leucobacter sp. JW-1. Science of the Total Environment 615: 78-86.
312. Chen, C.; Yan, X.; Yoza, B.A; Zhou, T.; Li, Y.; Zhan, Y.; Wang, Q,; Li, Q.X. 2018. Efficiencies and mechanisms of ZSM-5 zeolites loaded with cerium, iron, or manganese oxides for catalytic ozonation of nitrobenzene in water. Science of the Total Environment 612: 1424–1432. DOI: 10.1016/j.scitotenv.2017.09.019.
311. Clukey, K.E.; Lepczyk, C.A.; Balazs, G.H.; Work, T.M.; Li, Q.X.; Bachman, M.J.; Lynch, J. 2018. Persistent organic pollutants in fat of three species of Pacific pelagic longline caught sea turtles: Accumulation in relation to ingested plastic marine debris.Science of the Total Environment 610-611:402-411. DOI: 10.1016/j.scitotenv.2017.07.242
310. Pan, D.; Li, Q.X.; Lin, Z.; Chen, Z.; Tang, W.; Tan, H.; Pan, C.; Zeng, D. 2017. Interactions between salicylic acid and antioxidant enzymes tilting the balance of H2O2 from photorespiration in nontarget-crops under halosulfuron-methyl stress. Pesticide Biochemistry and Physiology 143: 214-223.
309. Cao, Z.; Zhang, W.; Ning, X.; Wang, B.; Liu, Y.; Li, Q.X. Development of monoclonal antibodies recognizing linear epitope: illustration by three Bacillus thuringiensis crystal proteins of genetically modified cotton, tobacco and maize. Journal of Agricultural and Food Chemistry 65(46): 10115-10122. 10.1021/acs.jafc.7b03426.
308. Wang, X.; Geng, A.; Dong, Y.; Fu, C.; Li, H.; Zhao, Y.; Li, Q.X.; Wang, F. 2017. Comparison of translocation and transformation from soil to rice and metabolism in rats for four arsenic species. Journal of Agricultural and Food Chemistry 65 (41): 8992–8998. DOI: 10.1021/acs.jafc.7b01779.
305. Wang, J.; Boesch, R.; Li, Q.X. 2017. A case study of air quality - pesticides and odorous phytochemicals on Kauai, Hawaii, USA.Chemosphere 189: 143-152. DOI: 10.1016/j.chemosphere.2017.09.045.
304. Chen, C.; Liang, J.; Yoza, B.A.; Li, Q.X.; Zhan, Y.; Wang, Q. 2017. Evaluation of an up-flow anaerobic sludge bed (UASB) reactor containing diatomite and maifanite for the improved treatment of petroleum wastewater. Bioresource Technology 243: 620-627. DOI: 10.1016/j.biortech.2017.06.171.
303. Wang, R.; Peng, J.; Li, Q.X.; Peng, Y-L. 2017. Phosphorylation-mediated regulatory networks in mycelia of Pyricularia oryzaerevealed by phosphoproteomic analyses. Molecular and Cellullar Proteomics 16: 1669-1682. DOI: 10.1074/mcp.M116.066670.
300. Wen, B.; Baker, M.R.; Zhao, H.; Cui, Z.; Li, Q.X. 2017. Expression and characterization of windmill palm tree (Trachycarpusfortunei) peroxidase by Pichia pastoris. Journal of Agricultural and Food Chemistry 65(23):4676–4682. DOI: 10.1021/acs.jafc.7b00318.
297. Wang, Y.; Zhu, M.; Jiang, E.; Hua, R.; Na, R.; Li, Q.X. 2017. A simple and rapid turn on ESIPT fluorescent probe for colorimetric and ratiometric detection of biothiols in living cells. Scientific Reports 7(1):4377. DOI: 10.1038/s41598-017-03901-8.
296. Lv, P.; Zhang, J.; Shi, T.; Dai, L.; Li, X.; Wu, X.; Li, X.; Tang, J.; Li, Q.X.; Hua, R. 2017. Procyanidolic oligomers enhance photodegradation of chlorothalonil in water via reductive dechlorination. Applied Catalysis B: Environmental 217: 137-143.
295. Chu, S.; Baker, M.R.; Leong, G.; Letcher, R.J.; Gee, S.J. Hammock, B.D.; Li, Q.X. 2017. Exploring Adduct Formation between Human Serum Albumin and Eleven Organophosphate Ester Flame Retardants and Plasticizers Using MALDI-TOF/TOF and LC-Q/TOF. Chemosphere 180: 169-177. DOI: 10.1016/j.chemosphere.2017.03.124.
293. Wu, L.; Cho, I.K.; Li, Y.; Zhang, G.; Li, Q.X. 2017. Evaluation of sources of irreproducibility of retention indices under programmed temperature gas chromatography conditions. Journal of Chromatography A 1495: 57-63. DOI: 10.1016/j.chroma.2017.03.009.
289. Fan, Y.; Feng, Q.; Lai, K.; Huang, W.; Zhang, C.; Li, Q.X. 2017. Toxic effects of indoxacarb enantiomers on the embryonic development and induction of apoptosis in zebrafish Larvae (Danio rerio). Environmental Toxicology 32 (1):7-16. DOI: 10.1002/tox.22207.
288. Liu, J.; Hua, R.; Lv, P.; Tang, J.; Wang, Y.; Cao, H.; Wu, X.; Li, Q.X. 2017. Novel hydrolytic de-methylthiolation of the s-triazine herbicide prometryn by Leucobacter sp. JW-1. Science of the Total Environment 579:115-123. DOI: 10.1016/j.scitotenv.2016.11.006.
279. Wu, L.; Duan, X.; Liu, C.; Zhang, G.; Li, Q.X. 2016. Phenomenon of dual- and single-retention behaviors of solutes and its validation by computational simulation in linear programmed temperature gas chromatography. Journal of Separation Science doi 10.1002/jssc.201600236
278. Liang, Z.; Zhang, B.; Su, W.W.; Williams, P.G.; Li, Q.X. 2016. C-Glycosylflavones alleviate tau phosphorylation and amyloid neurotoxicity through GSK3β inhibition. ACS Chemical Neuroscience doi: 10.1021/acschemneuro.6b00059
277. Baker, M.R.; Tabb, D.L.; Ching, T.; Zimmerman, L.J.; Sakharov, I.Y.; Li, Q.X. 2016. Site-specific N-glycosylation characterization of windmill palm tree peroxidase using novel tools for analysis of plant glycopeptide mass spectrometry data. Journal of Proteome Research 15(6): 2026-2038.
276. Hennessee, C.T.; Li Q.X. 2016. Effects of polycyclic aromatic hydrocarbon mixtures on degradation, gene expression, and metabolite production in four Mycobacterium species. Applied and Environmental Microbiology 82(11):3357-3369.
257. Chu, S.; Wang, J.; Leong, G.; Woodward, L.A.; Letcher, R.; Li, Q.X. 2015. Perfluoroalkyl sulfonates and carboxylic acids in liver, muscle and adipose tissues of black-footed albatross (Phoebastria nigripes) from Midway Island, North Pacific Ocean.Chemosphere 138:60-66.
245. Baker, M.R.; Zhao, H.; Sakharov, I.Y.; Li, Q.X. 2014. Amino acid sequence of anionic peroxidase from the windmill palm treeTrachycarpus fortunei. J. Agric. Food Chem. 62(49):11941–11948.
231. Cho, I.K.; Chang, C.L.; Li, Q.X. 2013. Diet-induced over-expression of flightless-I protein and its relation to flight dysfunction in Mediterranean fruit fly, Ceratitis capitata. PLoS ONE 8(12):e81099.
228. Seo, J.-S.; Keum, Y.-S.; Li, Q.X. 2013. Metabolomic and proteomic insights into carbaryl catabolism by Burkholderia sp. C3 and degradation of ten N-methylcarbamates. Biodegradation 24:795-811.
226. Baker, M.R.; Li, Q.X. 2013. Guanidination tryptic peptides without desalting for MALDI MS analysis. Anal. Chem.85(18):8873-8880.
218. Wu, L.; Chen M.X.; Chen, Y.; Li, Q.X. 2013. Determination and evaluation of gas holdup time with the quadratic equation model and comparison with nonlinear equation models for isothermal gas chromatography. J. Chromatogr. A 1297:196-203
202. Wang, D.; Atkinson, S.; Hoover-Miller A.; Shelver, W.; Li, Q.X. 2012. Organic halogenated contaminants in mother-fetus pairs of harbor seals (Phoca vitulina richardii) from Alaska, 2000-2002. J. Hazardous Materials 223-224:72-78.
197. Wang, J.; Chen, W.F.; Li, Q.X. 2012. Rapid identification and classification of Mycobacterium spp. using whole-cell protein barcodes and selected markers with MALDI TOF MS in comparison with multigene phylogenetic analysis. Anal. Chim. Acta716:133-137.
182. Seo, J.-S.; Keum, Y.-S.; Li, Q.X. 2011. Comparative protein and metabolite profiling revealed metabolic network in response to multiple environmental contaminants in Mycobacterium aromativorans JS19b1T. J. Agric. Food Chem. 59(7):2876-2882.
175. Wang, D.; Li, Q.X. 2010. Application of mass spectrometry in the analysis of polybrominated diphenyl ethers. Mass Spectrometry Reviews 29 (5):737-775.
153. Chang, C.L.; Cho, I.K.; Li, Q.X. 2009. Insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae. J. Economic Entomology 102(1): 203-209.
152. Hennessee, C.T.; Seo, J.-S.; Alvarez, A.M.; Li, Q.X. 2009. Isolation and characterization of five new polycyclic aromatic hydrocarbon-degrading species isolated from Hawaiian soils: Mycobacterium crocinum sp. nov., Mycobacterium pallens sp. nov., Mycobacterium rutilum sp. nov., Mycobacterium rufum sp. nov., and Mycobacterium aromaticivorans sp. nov. Int. J. Syst. Evol. Microbiol. 59(2):378-387.
122. Lee, S.-E.; Seo, J.S.; Keum, Y.-S.; Lee, K.-J.; Li, Q.X. 2007. Fluoranthene metabolism and associated proteins inMycobacterium sp. JS14. Proteomics 7(12):2059-2069.
082. Keum, Y.-S.; Li, Q.X. 2005. Reductive debromination of polybrominated diphenyl ethers by zero-valent Iron. Environ. Sci. Technol. 39(7):2280-2286.
081. Hou, S. et al. 2004. Genome sequence of the deep-sea γ-Proteobacterium Idiomarina Ioihiensis reveals amino acid fermentation as source of carbon and energy. Proc. Natl. Acad. Sci. USA 101(52):18036-18041.
075. Keum, Y.-S.; Li, Q.X. 2004. Copper dissociation as a mechanism of fungal laccase denaturation by humic acid. Appl. Microbiol. Biotech. 64: 588-592.
051. Chiu, Y.-W.; Li, Q.X.; Karu, A.E. 2001. Selective binding of polychlorinated biphenyl congeners by a monoclonal antibody: analysis by kinetic exclusion fluorescence immunoassay. Anal. Chem. 73(22):5477-5484.
046. Pellequer, J.L.; Zhao, B.; Kao, H.-I.; Bell, C.W.; Karu, A.E.; Li, K.; Li, Q.X.; Roberts, V.A. 2000. Stabilization of bound polycyclic aromatic hydrocarbons by a p-cation interaction. J. Mol. Biology 302(3):691-699.
045. David, M.D.; Campbell, S.; Li, Q.X. 2000. Pressurized fluid extraction of polar herbicides, and polar herbicides using in-situ derivatization. Anal. Chem. 72(15):3665-3670.
042. Thomas, S.D.; Li, Q.X. 2000. Immunoaffinity chromatography for the analysis of polycyclic aromatic hydrocarbons in corals.Environ. Sci. & Technol. 34(12), 2649-2654.
029. Guo, F.; Li, Q.X.; Alcantara-Licudine, J.P. 1999. A simple Na4EDTA-assisted sub/supercritical fluid extraction procedure for quantitative recovery of polar analytes in soil. Anal. Chem. 71:1309-1315.
027. Li, K.; Chen, R.; Zhao, B.; Liu, M.; Karu A.E.; Roberts, V.A.; Li, Q.X. 1999. Monoclonal antibody-based ELISAs for part-per-billion determination of polycyclic aromatic hydrocarbons: effects of haptens and formats on sensitivity and specificity. Anal. Chem. 71(2):302-309.
015. Li, Q.X.; Casida, J.E. 1994. Structure-activity studies leading to potent chloride channel blockers: 5e-tert-butyl-2-[4-(substituted-ethynyl)phenyl]-1,3-dithianes. Bioorg. & Med. Chem. 2(12):1423-1434.
005. Li, Q.X.; Gee, S.J.; McChesney, M.M.; Hammock, B.D.; Seiber, J.N. 1989. Comparison of an enzyme-linked immunosorbent assay and a gas chromatographic procedure for the determination of molinate residues. Anal. Chem. 61:818-823.
Languages : English, Chinese